Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation , the adult swine and rat islets were prepared 方法采用不同的膠原酶消化法及不連續密度梯度純化法。
So a lagre populations of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined with discontinuous percoll gradient centrifugation method 因此采用組合酶消化結合非連續percoll梯度離。 g法,可有效地從小鼠睪丸中分離到較多較純的存活精子細胞。
Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats , proliferative activities were compared in the cultued cells from the young , adult and aged rats 方法:應用梯度離心和貼壁培養分離大鼠肺泡型細胞,比較幼年、中年和老年大鼠培養肺泡型細胞的增殖能力。